PTBP1 Mouse Monoclonal Antibody [9A1] (EM1701-63)
Catalog# EM1701-63
Mouse Monoclonal to PTBP1
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
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Human
-
Rat
Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (EM1701-63) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-63) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-63) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.