PYK2 Recombinant Rabbit Monoclonal Antibody [SC06-15] (ET1610-82)

UNIPROT #

Q14289

PROTEIN NAME

PYK2

SYNONYMS

CADTK antibody;CAK-beta antibody;CAKB antibody;CAKbeta antibody;Calcium regulated non receptor proline rich tyrosine kinase antibody;Calcium-dependent tyrosine kinase antibody;Cell adhesion kinase beta antibody;E430023O05Rik antibody;EC 2.7.10.2 antibody;FADK 2 antibody;FADK2 antibody;FAK2 antibody;FAK2_HUMAN antibody;Focal adhesion kinase 2 antibody;MGC124628 antibody;PKB antibody;Proline-rich tyrosine kinase 2 antibody;Protein kinase B antibody;Protein Tyrosine Kinase 2 Beta antibody;Protein-tyrosine kinase 2-beta antibody;PTK antibody;PTK2B antibody;PTK2B protein tyrosine kinase 2 beta antibody;PYK2 antibody;RAFTK antibody;RAFTK2 antibody;Related adhesion focal tyrosine kinase antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

TISSUE SPECIFICITY

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.

SUBCELLULAR LOCATION

Cell membrane, Nucleus, Cytoplasm, perinuclear region, focal adhesion, lamellipodium, cell cortex.

FUNCTION

This gene encodes a cytoplasmic protein tyrosine kinase that is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. In addition, SOCE-induced Pyk2 activation mediates disassembly of endothelial adherens junctions, via tyrosine (Y1981-residue) phosphorylation of VE-PTP.