Ran Recombinant Rabbit Monoclonal Antibody [JE33-28] (HA721406)
Catalog# HA721406
Rabbit Monoclonal to Ran
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (HA721406) at 1/1,000 dilution.
Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: LoVo cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Lane 6: MDA-MB-231 cell lysate (15 µg/Lane)
Lane 7: MCF7 cell lysate (15 µg/Lane)
Lane 8: RAW264.7 cell lysate (15 µg/Lane)
Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
Lane 10: Rat thymus tissue lysate (30 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721406) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: LoVo cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Lane 6: MDA-MB-231 cell lysate (15 µg/Lane)
Lane 7: MCF7 cell lysate (15 µg/Lane)
Lane 8: RAW264.7 cell lysate (15 µg/Lane)
Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
Lane 10: Rat thymus tissue lysate (30 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721406) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.