RANKL Rabbit Polyclonal Antibody (HA500369)
Catalog# HA500369
Rabbit Polyclonal to RANKL
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Mouse spleen tissue lysate (20 µg/Lane)
Lane 3: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500369) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
RANKL Rabbit Polyclonal Antibody (HA500369)
Immunogen
Recombinant protein within human rankl aa 100-317.
Host
Rabbit
Positive Control
Hela cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
RANKL
SYNONYMS
CD254 antibody;hRANKL2 antibody;ODF antibody;OPGL antibody;OPTB2 antibody;Osteoclast differentiation factor antibody;Osteoprotegerin ligand antibody;Rank Ligand antibody;RANKL antibody;Receptor activator of nuclear factor kappa B ligand antibody;Receptor activator of nuclear factor kappa-B ligand antibody;sOdf antibody;TNF related activation induced cytokine antibody;TNF-related activation-induced cytokine antibody;TNF11_HUMAN antibody;TNFSF 11 antibody;Tnfsf11 antibody;TRANCE antibody;Tumor necrosis factor (ligand) superfamily member 11 antibody;Tumor necrosis factor ligand superfamily member 11 antibody;Tumor necrosis factor ligand superfamily member 11, soluble form antibody
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm.
FUNCTION
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
CITATIONS
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Suppression of IRF9 Promotes Osteoclast Differentiation by Decreased Ferroptosis via STAT3 Activation
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Mouse
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Citation
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Parathyroid hormone increases alveolar bone homoeostasis during orthodontic tooth movement in rats with periodontitis via crosstalk between STAT3 and β-catenin
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IHC
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Rat
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Citation
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Huang Yucheng,et al
Si-Zhi Wan regulates osteoclast autophagy in osteoporosis through the AMPK signaling pathway to attenuate osteoclastogenesis
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WB
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Mouse
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Citation
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