RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15] (HA721273)
Catalog# HA721273
Rabbit Monoclonal to RbBP5
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: A431 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: 293 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: K562 cell lysate
Lane 8: N2A cell lysate
Lane 9: PC-12 cell lysate
Lane 10: RAW264.7 cell lysate
Lysates/proteins at 25 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: SW480 cell lysate
Lane 2: A431 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: 293 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: K562 cell lysate
Lane 8: N2A cell lysate
Lane 9: PC-12 cell lysate
Lane 10: RAW264.7 cell lysate
Lysates/proteins at 25 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.