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Restriction enzyme FokI(Internal) Rabbit Polyclonal Antibody (R1501-4)

Price: Regular price $315.00

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Catalog# R1501-4

Rabbit Polyclonal to Restriction enzyme FokI(Internal)

Tested for

  • WB

Reacts with

  • Planomicrobium okeanokoites

Specifications

Product Type

Rabbit polyclonal primary

Product Name

Restriction enzyme FokI(Internal) Rabbit Polyclonal Antibody (R1501-4)

Immunogen

Synthetic peptide within planomicrobium okeanokoites fokir aa 444-456.

Host

Rabbit

Positive Control

Purified restriction enzyme FokI

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3073314

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Molecular Weight

Predicted band size: 66 kDa

Isotype

IgG

TARGET

UNIPROT #

P14870

PROTEIN NAME

Restriction enzyme FokI(Internal)

FUNCTION

The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.

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