Ribonuclease 3 Mouse Monoclonal Antibody [5G4] (EM1801-17)

UNIPROT #

P12724

PROTEIN NAME

Ribonuclease 3

SYNONYMS

Cytotoxic ribonuclease antibody;ECP antibody;ECP_HUMAN antibody;Eosinophil cationic protein antibody;OTTHUMP00000164017 antibody;Ribonuclease 3 antibody;Ribonuclease, RNase A family, 3 antibody;RNase 3 antibody;RNASE3 antibody;RNS3 antibody

SEQUENCE SIMILARITIES

Belongs to the pancreatic ribonuclease family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. The protein exhibits antimicrobial activity against pathogenic bacteria. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. ECP is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Mature ECP is cytotoxic to human bronchial epithelial (BEAS-2B) cells by specific binding to cell surface heparan sulfate proteoglycans (HSPGs) followed by endocytosis. ECP triggers apoptosis by caspase-8 activation through mitochondria-independent pathway. Increases in chromatin condensation, sub-G1 population, PARP cleavage, and DNA fragmentation indicate that ECP induces apoptosis in human bronchial epithelial (BEAS-2B) cells.