RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11] (HA722221)
Catalog# HA722221
Rabbit Monoclonal to RNase L
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of RNase L on different lysates with Rabbit anti-RNase L antibody (HA722221) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75 kDa
Exposure time: Lane 1-2: 6 seconds; Lane 3-4: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722221) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75 kDa
Exposure time: Lane 1-2: 6 seconds; Lane 3-4: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722221) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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