RUNX2 Recombinant Rabbit Monoclonal Antibody [SD208-0] (ET1612-47)
Catalog# ET1612-47
Rabbit Monoclonal to RUNX2
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IF-Cell
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IF-Tissue
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IHC-P
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WB
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FC
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IP
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mIHC
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Human
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Mouse
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Rat
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RUNX2 antibody (ET1612-47) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
RUNX2 Recombinant Rabbit Monoclonal Antibody [SD208-0] (ET1612-47)
Immunogen
Recombinant protein within human 300-450.
Host
Rabbit
Positive Control
MDA-MB-231 cell lysate, Saos-2 cell lysate, NIH/3T3 cell lysate, Saos-2, SW480, human tonsil tissue, human colon tissue, human spleen tissue, mouse bone tissue, rat maxilla tissue, C2C12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD208-0
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IF-Cell
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1:1,000-1:5,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:5,000
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IP
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1-2μg/sample
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mIHC
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
RUNX2
SYNONYMS
Acute myeloid leukemia 3 protein antibody;Alpha subunit 1 antibody;AML3 antibody;CBF alpha 1 antibody;CBF-alpha-1 antibody;CBFA1 antibody;CCD antibody;CCD1 antibody;Cleidocranial dysplasia 1 antibody;Core binding factor antibody;Core binding factor runt domain alpha subunit 1 antibody;Core binding factor subunit alpha 1 antibody;Core-binding factor subunit alpha-1 antibody;MGC120022 antibody;MGC120023 antibody;Oncogene AML 3 antibody;Oncogene AML-3 antibody;OSF 2 antibody;OSF-2 antibody;OSF2 antibody;Osteoblast specific transcription factor 2 antibody;Osteoblast-specific transcription factor 2 antibody;OTTHUMP00000016533 antibody;PEA2 alpha A antibody;PEA2-alpha A antibody;PEA2aA antibody;PEBP2 alpha A antibody;PEBP2-alpha A antibody;PEBP2A1 antibody;PEBP2A2 antibody;PEBP2aA antibody;PEBP2aA1 antibody;Polyomavirus enhancer binding protein 2 alpha A subunit antibody;Polyomavirus enhancer-binding protein 2 alpha A subunit antibody;Runt domain antibody;Runt related transcription factor 2 antibody;Runt-related transcription factor 2 antibody;RUNX2 antibody;RUNX2_HUMAN antibody;SL3 3 enhancer factor 1 alpha A subunit antibody;SL3-3 enhancer factor 1 alpha A subunit antibody;SL3/AKV core binding factor alpha A subunit antibody;SL3/AKV core-binding factor alpha A subunit antibody
TISSUE SPECIFICITY
Specifically expressed in osteoblasts.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated; probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation (By similarity). Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature.
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