SCA-1 Rabbit Polyclonal Antibody (0804-10)
Catalog# 0804-10
Rabbit Polyclonal to SCA-1
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WB
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IF-Cell
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Mouse
Lane 1: Mouse kidney tissue lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SCA-1 Rabbit Polyclonal Antibody (0804-10)
Immunogen
Synthetic peptide within mouse sca-1 aa 51-94.
Host
Rabbit
Positive Control
Mouse kidney tissue lysate, NIH/3T3 cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 14 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SCA-1
SYNONYMS
Ly-6A.2/Ly-6E. antibody;Ly-6A.2/Ly-6E.1 antibody;Ly6a antibody;LY6A_MOUSE antibody;Ly6al antibody;Lymphocyte antigen 6A-2/6E-1 antibody;Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody;SCA-1 antibody;Stem cell antigen 1 antibody;T cell activating protein antibody;T-cell-activating protein antibody;TAP antibody
TISSUE SPECIFICITY
Widely expressed.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated. Not N-glycosylated.; Not phosphorylated.
SUBCELLULAR LOCATION
Cell membrane
FUNCTION
Sca-1 stands for "Stem cells antigen-1" (official gene symbol: Ly6a). It consist of 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein (GPI-AP) of the LY6 gene family. It is the common biological marker used to identify hematopoitic stem cell (HSC) along with other markers. Sca-1 has a regenerative role in cardiac repair: Host cells with specific Sca-1+CD31− markers arise upon myocardial infarction, with evidence of expression of Sca-1 protein. Sca-1 plays a role in hematopoietic progenitor/stem cell lineage fate and c-kit expression. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.
CITATIONS
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Wang, Y., Qin, W. Y., Wang, Q., Liu, X. N., Li, X. H., Ye, X. Q., Bai, Y., Zhang, Y., Liu, P., Wang, X. L., Zhou, Y. H., Yuan, H. P., & Shao, Z. B.
Young Sca-1+ bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization
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WB
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Mouse
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Citation
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