SCGN Recombinant Rabbit Monoclonal Antibody [JE60-48] (HA721891)
Catalog# HA721891
Rabbit Monoclonal to SCGN
Tested for
-
WB
-
IHC-P
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IF-Cell
-
FC
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SCGN on different lysates with Rabbit anti-SCGN antibody (HA721891) at 1/2,000 dilution.
Lane 1: TT cell lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse eyeball tissue lysate
Lane 4: Mouse pancreas tissue lysate
Lane 5: Rat eyeball tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721891) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TT cell lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse eyeball tissue lysate
Lane 4: Mouse pancreas tissue lysate
Lane 5: Rat eyeball tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721891) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.