SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-90] (HA721748)
Catalog# HA721748
Rabbit Monoclonal to SHARP2
Tested for
-
WB
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IHC-P
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IF-Cell
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA721748) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: 293T cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46/50 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: 293T cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46/50 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.