SHP2 Recombinant Rabbit Monoclonal Antibody [SN72-02] (ET1611-35)
Catalog# ET1611-35
Rabbit Monoclonal to SHP2
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WB
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IP
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IHC-P
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SHP2 Recombinant Rabbit Monoclonal Antibody [SN72-02] (ET1611-35)
Immunogen
Synthetic peptide within n-terminal human shp2.
Host
Rabbit
Positive Control
K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, rat heart tissue lysate, human tonsil tissue, mouse testis tissue, rat testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN72-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IP
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Use at an assay dependent concentration.
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IHC-P
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SHP2
SYNONYMS
BPTP3 antibody;CFC antibody;JMML antibody;METCDS antibody;MGC14433 antibody;NS1 antibody;OTTHUMP00000166107 antibody;OTTHUMP00000166108 antibody;Protein tyrosine phosphatase 2 antibody;Protein tyrosine phosphatase 2C antibody;Protein tyrosine phosphatase non receptor type 11 antibody;Protein-tyrosine phosphatase 1D antibody;Protein-tyrosine phosphatase 2C antibody;PTN11_HUMAN antibody;PTP-1D antibody;PTP-2C antibody;PTP1D antibody;PTP2C antibody;PTPN11 antibody;SAP2 antibody;SH-PTP2 antibody;SH-PTP3 antibody;SH2 domain containing protein tyrosine phosphatase 2 antibody;SHP 2 antibody;SHP-2 antibody;Shp2 antibody;SHPTP2 antibody;SHPTP3 antibody;Syp antibody;Tyrosine-protein phosphatase non-receptor type 11 antibody
SEQUENCE SIMILARITIES
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
TISSUE SPECIFICITY
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus .Positively regulates MAPK signal transduction pathway.Dephosphorylates GAB1, ARHGAP35 and EGFR.Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity.Dephosphorylates CDC73.Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification.
CITATIONS
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Ajuforrestin A, an Abietane Diterpenoid from Ajuga ovalifolia var. calanthe, Induces A549 Cell Apoptosis by Targeting SHP2
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WB
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Human
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Citation
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