Smad2 Recombinant Rabbit Monoclonal Antibody [SP06-05] (ET1604-22)
Catalog# ET1604-22
Rabbit Monoclonal to Smad2
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WB
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IF-Cell
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 1 minute 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-22) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Smad2 Recombinant Rabbit Monoclonal Antibody [SP06-05] (ET1604-22)
Immunogen
Synthetic peptide within human smad2 aa 220-270.
Host
Rabbit
Positive Control
HeLa cell lysate, HT-29 cell lysate, Jurkat cell lysate, HL-60 cell lysate, C2C12 cell lysate, mouse lung tissue lysate, mouse placenta tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, HepG2, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP06-05
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 52 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Smad2
SYNONYMS
Drosophila, homolog of, MADR2 antibody;hMAD-2 antibody;HsMAD2 antibody;JV18 antibody;JV18-1 antibody;JV181 antibody;MAD antibody;MAD homolog 2 antibody;MAD Related Protein 2 antibody;Mad-related protein 2 antibody;MADH2 antibody;MADR2 antibody;MGC22139 antibody;MGC34440 antibody;Mother against DPP homolog 2 antibody;Mothers against decapentaplegic homolog 2 antibody;Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody;Mothers against DPP homolog 2 antibody;OTTHUMP00000163489 antibody;Sma and Mad related protein 2 antibody;Sma- and Mad-related protein 2 MAD antibody;SMAD 2 antibody;SMAD family member 2 antibody;SMAD, mothers against DPP homolog 2 antibody;SMAD2 antibody;SMAD2_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the dwarfin/SMAD family.
TISSUE SPECIFICITY
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.; In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity).; Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
CITATIONS
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Profilin 2 promotes growth, metastasis, and angiogenesis of small cell lung cancer through cancer-derived exosomes
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WB
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Human
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Citation
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TBX20 Contributes to Balancing the Differentiation of Perivascular Adipose-Derived Stem Cells to Vascular Lineages and Neointimal Hyperplasia
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WB
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Mouse
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Citation
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Hu, X., Chen, M., Cao, X., Yuan, X., Zhang, F., & Ding, W.
TGF-β-Containing Small Extracellular Vesicles From PM2.5-Activated Macrophages Induces Cardiotoxicity
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WB
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Mouse
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Citation
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Meng Lingjie,et al
The combination of paeoniflorin and metformin synergistically inhibits the progression of liver fibrosis in mice
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WB
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Mouse
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Citation
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Yirong Wu, Siyao Zhan, Lian Chen, Mingrui Sun, Miaofu Li, Xuanting Mou, Zhen Zhang, Linhao Xu, Yizhou Xu
TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisation
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WB
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Mouse
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Citation
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Wu Bibo,et al
PD-1 Inhibitor Aggravate Irradiation-Induced Myocardial Fibrosis by Regulating TGF-β1/Smads Signaling Pathway via GSDMD-Mediated Pyroptosis
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WB
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Mouse
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Citation
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Ouyang, Z. Q., Shao, L. S., Wang, W. P., Ke, T. F., Chen, D., Zheng, G. R., Duan, X. R., Chu, J. X., Zhu, Y., Yang, L., Shan, H. Y., Huang, L., & Liao, C. D.
Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis
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WB
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Rat
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Citation
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Xue, F., Zhao, Z., Gu, Y., Han, J., Ye, K., & Zhang, Y.
7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. eLife, 10, e64872.
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WB
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Rat
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Citation
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CD44 mediates hyaluronan to promote the differentiation of human amniotic mesenchymal stem cells into chondrocytes
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WB
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Human
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Citation
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