Skip to content

Contact

(857) 353-6600

orders@huabio.com

Smad3 Recombinant Rabbit Monoclonal Antibody [SY25-01] (ET1607-41)

Price: Regular price $385.00

Volume

Bulk Order Quote   |   Customization Request

Catalog# ET1607-41

Rabbit Monoclonal to Smad3

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

Select your chosen country/region

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Smad3 Recombinant Rabbit Monoclonal Antibody [SY25-01] (ET1607-41)

Immunogen

Synthetic peptide within human smad3 aa 181-230 / 425.

Host

Rabbit

Positive Control

HeLa cell lysate, A549 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, bEnd.3 cell lysate, C6 cell lysate, Wild-type HaCaT whole cell lysate, A549, human lung carcinoma tissue, human breast carcinoma tissue, mouse liver tissue, mouse brain tissue, rat testis tissue, HeLa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY25-01

RRID

AB_3069765

APPLICATION DILUTION

  • WB

  • 1:5,000-1:20,000

  • IF-Cell

  • 1:500

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 48 kDa

Isotype

IgG

TARGET

UNIPROT #

P84022

PROTEIN NAME

Smad3

SYNONYMS

DKFZP586N0721 antibody;DKFZp686J10186 antibody;hMAD 3 antibody;hMAD-3 antibody;hSMAD3 antibody;HSPC193 antibody;HST17436 antibody;JV15 2 antibody;JV15-2 antibody;JV152 antibody;LDS1C antibody;LDS3 antibody;MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody;MAD homolog 3 antibody;Mad homolog JV15 2 antibody;Mad protein homolog antibody;MAD, mothers against decapentaplegic homolog 3 antibody;Mad3 antibody;MADH 3 antibody;MADH3 antibody;MGC60396 antibody;Mothers against decapentaplegic homolog 3 antibody;Mothers against DPP homolog 3 antibody;SMA and MAD related protein 3 antibody;SMAD 3 antibody;SMAD antibody;SMAD family member 3 antibody;SMAD, mothers against DPP homolog 3 antibody;Smad3 antibody;SMAD3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol. Maximum phosphorylation at the G(1)/S junction. Also phosphorylated on serine residues in the C-terminal SXS motif by TGFBR1 and ACVR1. TGFBR1-mediated phosphorylation at these C-terminal sites is required for interaction with SMAD4, nuclear location and transactivational activity, and appears to be a prerequisite for the TGF-beta mediated phosphorylation in the linker region. Dephosphorylated in the C-terminal SXS motif by PPM1A. This dephosphorylation disrupts the interaction with SMAD4, promotes nuclear export and terminates TGF-beta-mediated signaling. Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Phosphorylated by PDPK1.; Acetylation in the nucleus by EP300 in the MH2 domain regulates positively its transcriptional activity and is enhanced by TGF-beta.; Poly-ADP-ribosylated by PARP1 and PARP2. ADP-ribosylation negatively regulates SMAD3 transcriptional responses during the course of TGF-beta signaling.; Ubiquitinated. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover (By similarity). Undergoes STUB1-mediated ubiquitination and degradation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

CITATIONS

  • Effect of fecal microbiota transplantation on the TGF-β1/Smad signaling pathway in rats with TNBS-induced colitis

  • Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.

    Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways

  • Ping Fu; Liang Ma

    Pterostilbene, a bioactive component of blueberries, alleviates renal fibrosis in a severe mouse model of hyperuricemic nephropathy. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 109, 1802–1808.

  • Ouyang, Z. Q., Shao, L. S., Wang, W. P., Ke, T. F., Chen, D., Zheng, G. R., Duan, X. R., Chu, J. X., Zhu, Y., Yang, L., Shan, H. Y., Huang, L., & Liao, C. D.

    Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis

  • Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

  • Hu, X., Chen, M., Cao, X., Yuan, X., Zhang, F., & Ding, W.

    TGF-β-Containing Small Extracellular Vesicles From PM2.5-Activated Macrophages Induces Cardiotoxicity

  • Ren, Q., Tao, S., Guo, F., Wang, B., Yang, L., Ma, L., & Fu, P.

    Natural flavonol fisetin attenuated hyperuricemic nephropathy via inhibiting IL-6/JAK2/STAT3 and TGF-β/SMAD3 signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology, 87, 153552.

  • Qing Huang ,et al

    PCSK9 inhibitor attenuates cardiac fibrosis in reperfusion injury rat by suppressing inflammatory response and TGF-β1/Smad3 pathway

  • Fibulin-3 regulates the inhibitory effect of TNF-α on chondrocyte differentiation partially via the TGF-β/Smad3 signaling pathway

  • Wu Bibo,et al

    PD-1 Inhibitor Aggravate Irradiation-Induced Myocardial Fibrosis by Regulating TGF-β1/Smads Signaling Pathway via GSDMD-Mediated Pyroptosis

  • Treatment with a PPAR-γ Agonist Protects Against Hyperuricemic Nephropathy in a Rat Model

  • Monocyte-derived immature dendritic cells negatively regulate hepatic stellate cells in vitro by secreting IL-10

  • TBX20 Contributes to Balancing the Differentiation of Perivascular Adipose-Derived Stem Cells to Vascular Lineages and Neointimal Hyperplasia

  • Li, Y., Guo, F., Huang, R., Ma, L., & Fu, P.

    Natural flavonoid pectolinarigenin alleviated kidney fibrosis via inhibiting the activation of TGFβ/SMAD3 and JAK2/STAT3 signaling. International immunopharmacology, 91, 107279.

  • Dandan Wu,et al

    RRM2 Regulates Hepatocellular Carcinoma Progression Through Activation of TGF-β/Smad Signaling and Hepatitis B Virus Transcription

  • Yirong Wu, Siyao Zhan, Lian Chen, Mingrui Sun, Miaofu Li, Xuanting Mou, Zhen Zhang, Linhao Xu, Yizhou Xu

    TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisation

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

Search

Main menu

Your cart is currently empty.
Shop now