Smad4 Rabbit Polyclonal Antibody (ER1706-90)

UNIPROT #

Q13485

PROTEIN NAME

Smad4

SYNONYMS

(Small) Mothers Against Decapentaplegic antibody;Deleted in Pancreatic Carcinoma 4 antibody;Deleted in Pancreatic Carcinoma antibody;Deleted in pancreatic carcinoma locus 4 antibody;Deletion target in pancreatic carcinoma 4 antibody;DPC 4 antibody;DPC4 antibody;hSMAD4 antibody;JIP antibody;MAD homolog 4 antibody;MAD mothers against decapentaplegic Drosophila homolog 4 antibody;MAD mothers against decapentaplegic homolog 4 antibody;MADH 4 antibody;MADH4 antibody;Med antibody;Medea antibody;Mothers against decapentaplegic homolog 4 antibody;Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody;Mothers against DPP homolog 4 antibody;MYHRS antibody;OTTHUMP00000163548 antibody;SMA- and MAD-related protein 4 antibody;SMAD 4 antibody;SMAD family member 4 antibody;SMAD mothers against DPP homolog 4 antibody;SMAD4 antibody;SMAD4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PDPK1.; Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.