SMARCA2 Rabbit Polyclonal Antibody (ER65406)
Catalog# ER65406
Rabbit Polyclonal to SMARCA2
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WB
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IF-Cell
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IHC-P
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FC
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Human
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65406) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SMARCA2 Rabbit Polyclonal Antibody (ER65406)
Immunogen
Synthetic peptide within human smarca2 aa 231-280 / 1,590.
Host
Rabbit
Positive Control
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, HeLa, MCF7, human colon cancer tissue, human prostate cancer tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:50
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 181 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SMARCA2
SYNONYMS
ATP dependent helicase SMARCA2 antibody;ATP-dependent helicase SMARCA2 antibody;BAF190 antibody;BAF190B antibody;BRG1-associated factor 190B antibody;BRM antibody;FLJ36757 antibody;Global transcription activator homologous sequence antibody;hBRM antibody;hSNF2a antibody;MGC74511 antibody;Possible global transcription activator SNF2L2 antibody;Probable global transcription activator SNF2L2 antibody;Protein brahma homolog antibody;SMARCA2 antibody;SMCA2_HUMAN antibody;SNF2 alpha antibody;SNF2 like 2 antibody;SNF2-alpha antibody;SNF2/SWI2 like protein 2 antibody;SNF2L2 antibody;SNF2LA antibody;Sth1p antibody;Sucrose nonfermenting 2 like protein 2 antibody;SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
CITATIONS
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Jiang, H., Bian, W., Sui, Y., Li, H., Zhao, H., Wang, W., & Li, X.
FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJ
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WB
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Human
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Citation
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