SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-62] (HA721450)
Catalog# HA721450
Rabbit Monoclonal to SMC2
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: HT-29 cell lysate (30 µg/Lane)
Lane 4: HepG2 cell lysate (30 µg/Lane)
Lane 5: A431 cell lysate (30 µg/Lane)
Lane 6: SH-SY5Y cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: Raji cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (24 µg/Lane)
Predicted band size: 136 kDa
Observed band size: 136 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721450) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: HT-29 cell lysate (30 µg/Lane)
Lane 4: HepG2 cell lysate (30 µg/Lane)
Lane 5: A431 cell lysate (30 µg/Lane)
Lane 6: SH-SY5Y cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: Raji cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (24 µg/Lane)
Predicted band size: 136 kDa
Observed band size: 136 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721450) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.