SNAP25 Recombinant Rabbit Monoclonal Antibody [JE45-86] (HA721892)
Catalog# HA721892
Rabbit Monoclonal to SNAP25
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (negative) (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721892) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SNAP25 Recombinant Rabbit Monoclonal Antibody [JE45-86] (HA721892)
Immunogen
Recombinant protein within human snap25 aa 51-150 / 206.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, human brain tissue lysate, SH-SY5Y, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE45-86
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IHC-P
-
1:500-1:5,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SNAP25
SYNONYMS
bA416N4.2 antibody;Bdr antibody;CMS18 antibody;dJ1068F16.2 antibody;FLJ23079 antibody;HGNC:11132 antibody;MGC105414 antibody;MGC139754 antibody;Resistance to inhibitors of cholinesterase 4 homolog antibody;RIC 4 antibody;RIC-4 antibody;RIC4 antibody;SEC 9 antibody;SEC9 antibody;SNAP 25 antibody;SNAP antibody;SNAP-25 antibody;SNAP-25B antibody;SNAP25 antibody;SNP 25 antibody;SNP25 antibody;SNP25_HUMAN antibody;sp antibody;SUP antibody;Super protein antibody;Synaptosomal associated 25 kDa protein antibody;Synaptosomal associated protein antibody;Synaptosomal associated protein 25 antibody;Synaptosomal associated protein 25kDa antibody;Synaptosomal-associated 25 kDa protein antibody;Synaptosomal-associated protein 25 antibody;Synaptosomal-associated protein antibody;Synaptosomal-associated protein, 25-KD antibody
SUBCELLULAR LOCATION
Cytoplasm, perinuclear region. Cell membrane.
FUNCTION
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. When cloned and expressed in E.coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster. Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
CITATIONS
-
Xie Jiaojiao,et al
Fibroblast growth factor 21 enhances learning and memory performance in mice by regulating hippocampal L-lactate homeostasis
-
WB
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.