Sortilin Recombinant Rabbit Monoclonal Antibody [JE60-00] (HA722070)
Catalog# HA722070
Rabbit Monoclonal to Sortilin
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (HA722070) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (40 µg/Lane)
Lane 3: Human kidney tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Human lung tissue lysate (40 µg/Lane)
Lane 3: Human kidney tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.