SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14] (ET1704-76)
Catalog# ET1704-76
Rabbit Monoclonal to SPHK1
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WB
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Human
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Mouse
Western blot analysis of SPHK1 on different lysates with Rabbit anti-SPHK1 antibody (ET1704-76) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: RAW264.7 cell lysate (20 µg/Lane)
Lane 5: Mouse lung tissue lysate (30 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 43-51 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-76) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14] (ET1704-76)
Immunogen
Synthetic peptide within human sphk1 aa 1-200 / 384.
Host
Rabbit
Positive Control
HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, Mouse lung tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA31-14
RRID
APPLICATION DILUTION
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WB
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SPHK1
SYNONYMS
SK 1 antibody;SK1 antibody;Sphingosine kinase 1 antibody;SPHK 1 antibody;SPHK antibody;Sphk1 antibody;SPHK1_HUMAN antibody;SPK antibody;SPK 1 antibody
TISSUE SPECIFICITY
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).
SUBCELLULAR LOCATION
Cytoplasm. Nucleus. Membrane.
FUNCTION
Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFα. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.
CITATIONS
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Ye, L., Song, J., Zheng, Y., Zhong, M., Liu, J., Zhu, D., & Hu, S.
New mechanism for mesenchymal stem cell microvesicle to restore lung permeability: intracellular S1P signaling pathway independent of S1P receptor-1
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WB
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Human
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Citation
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