SRC Rabbit Polyclonal Antibody (R1312-9)

UNIPROT #

P12931

PROTEIN NAME

SRC

SYNONYMS

ASV antibody;Avian sarcoma virus antibody;c SRC antibody;CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody;cSrc antibody;EC 2.7.10.2 antibody;Neuronal CSRC tyrosine specific protein kinase antibody;Neuronal SRC antibody;Oncogene SRC antibody;OTTHUMP00000174476 antibody;OTTHUMP00000174477 antibody;p60 Src antibody;p60-Src antibody;p60Src antibody;pp60c src antibody;pp60c-src antibody;pp60csrc antibody;Proto oncogene tyrosine protein kinase Src antibody;Proto-oncogene c-Src antibody;Proto-oncogene tyrosine-protein kinase Src antibody;Protooncogene SRC antibody;Protooncogene SRC Rous sarcoma antibody;Src antibody;SRC Oncogene antibody;SRC proto oncogene non receptor tyrosine kinase antibody;SRC_HUMAN antibody;SRC1 antibody;Tyrosine kinase pp60c src antibody;Tyrosine protein kinase SRC 1 antibody;Tyrosine protein kinase SRC1 antibody;v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody;V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody;v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

TISSUE SPECIFICITY

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.

POST-TRANSLATIONAL MODIFICATION

Myristoylated at Gly-2, and this is essential for targeting to membranes.; Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation. CDK5-mediated phosphorylation at Ser-75 targets SRC to ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. Phosphorylated by PTK2/FAK1; this enhances kinase activity. Phosphorylated by PTK2B/PYK2; this enhances kinase activity.; S-nitrosylation is important for activation of its kinase activity.; Ubiquitinated in response to CDK5-mediated phosphorylation. Ubiquitination mediated by CBLC requires SRC autophosphorylation at Tyr-419 and may lead to lysosomal degradation.

SUBCELLULAR LOCATION

Cell membrane, nucleus, cytoplasm

FUNCTION

Src (pronounced "sarc" as it is short for sarcoma) is a proto-oncogene encoding a tyrosine kinase. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. c-Src is made up of 6 functional regions: Src homology (SH) 4 domain (SH4 domain), unique region, SH3 domain, SH2 domain, catalytic domain and short regulatory tail. When Src is inactive, the phosphorylated tyrosine group at the 527 position interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. The activation of c-Src causes the dephosphorylation of the tyrosine 527 which causes the structure to be destabilized and then result in the opening up of the SH3, SH2 and the kinase domains and autophosphorylation of tyrosine 416. The activation of the c-Src pathway has been observed in about 50% of tumors from colon, liver, lung, breast and the pancreas.