SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] (ET7109-32)
Catalog# ET7109-32
Rabbit Monoclonal to SRP54
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WB
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: MCF-7 cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SRP54 Recombinant Rabbit Monoclonal Antibody [JE43-25] (ET7109-32)
Immunogen
Synthetic peptide within human srp54 aa 441-490 / 504.
Host
Rabbit
Positive Control
MCF-7 cell lysate, HepG2 cell lysate, rat brain tissue, human breast carcinoma tissue, human kidney tissue, mouse brain tissue, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE43-25
RRID
APPLICATION DILUTION
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WB
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1:500
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 56 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SRP54
SYNONYMS
Signal recognition particle 54 kDa protein antibody;Signal recognition particle 54kDa antibody;SRP 54 antibody;SRP54 antibody;SRP54_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the GTP-binding SRP family. SRP54 subfamily.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
SRP54, a component of the signal recognition particle (SRP), recognizes the signal peptide of secretory proteins and interacts with the signal recognition particle receptor to target the ribosome and the associated nascent chain to the endoplasmic reticulum. By functional analysis showed that SRP54 binds to SRP RNA via the M-domain, but only in the presence of RNA-bound SRP19, and that it associates with the signal peptide of nascent polypeptide chains. RNA interaction requires the presence of a loop in the C-terminal M-domain. Signal peptide recognition most likely involves methionine-rich loops. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a (602326) and L35. When SRP54 contacts the signal recognition particle receptor (182180), SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.
CITATIONS
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Turnip mosaic virus P1 suppresses JA biosynthesis by degrading cpSRP54 that delivers AOCs onto the thylakoid membrane to facilitate viral infection
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WB
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Virens
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Citation
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