Strep-Tag II Mouse Monoclonal Antibody [A5D11] (HA600038)
Catalog# HA600038
Mouse Monoclonal to Strep-Tag II
Tested for
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WB
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IF-Cell
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FC
Reacts with
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Species independent
Western blot analysis of Strep-Tag II on different lysates with Mouse anti-Strep-Tag II antibody (HA600038) at 1/1,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate
Lane 3: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600038) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T-NT cell lysate
Lane 2: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate
Lane 3: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600038) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.