SUPT5H Recombinant Rabbit Monoclonal Antibody [JE55-25] (ET7111-01)

UNIPROT #

O00267

PROTEIN NAME

SUPT5H

SYNONYMS

DRB sensitivity inducing factor large subunit antibody;DRB sensitivity-inducing factor 160 kDa subunit antibody;DRB sensitivity-inducing factor large subunit antibody;DSIF large subunit antibody;DSIF p160 antibody;FLJ34157 antibody;hSPT 5 antibody;hSPT5 antibody;SPT 5 antibody;SPT 5H antibody;SPT5 antibody;SPT5H antibody;SPT5H_HUMAN antibody;Suppressor of Ty 5 homolog antibody;Suppressor of Ty 5 homolog (S. cerevisiae) antibody;SUPT 5H antibody;supt5h antibody;Tat cotransactivator 1 protein antibody;Tat CT1 antibody;Tat CT1 protein antibody;Tat-cotransactivator 1 protein antibody;Tat-CT1 protein antibody;Transcription elongation factor SPT 5 antibody;Transcription elongation factor spt5 antibody;Transcription factor Tat CT1 antibody

SEQUENCE SIMILARITIES

Belongs to the SPT5 family.

TISSUE SPECIFICITY

Ubiquitously expressed.

POST-TRANSLATIONAL MODIFICATION

Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.; Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.