Survivin Recombinant Rabbit Monoclonal Antibody [SP07-06] (ET1604-34)

UNIPROT #

O15392

PROTEIN NAME

Survivin

SYNONYMS

API4 antibody;Apoptosis inhibitor 4 antibody;Apoptosis inhibitor survivin antibody;Apoptosis inhibitor4 antibody;Baculoviral IAP repeat containing 5 antibody;Baculoviral IAP repeat containing protein 5 antibody;Baculoviral IAP repeat-containing protein 5 antibody;BIRC 5 antibody;BIRC5 antibody;BIRC5_HUMAN antibody;EPR 1 antibody;IAP4 antibody;Survivin variant 3 alpha antibody;SVV antibody;TIAP antibody

SEQUENCE SIMILARITIES

Belongs to the IAP family.

TISSUE SPECIFICITY

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level).

DEVELOPMENTAL STAGE

Expression is cell cycle-dependent and peaks at mitosis.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.; In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities. Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity. Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.; Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Chromosome, Midbody.

FUNCTION

The baculovirus protein p35 inhibits virally-induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivin-microtubule interactions occurs.