SUV39H2 Recombinant Rabbit Monoclonal Antibody [JG36-55] (ET7108-37)
Catalog# ET7108-37
Rabbit Monoclonal to SUV39H2
-
WB
-
IHC-P
-
IP
-
Human
-
Mouse
-
Rat
Lane 1: U-2 OS cell lysate
Lane 2: A549 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HEK-293 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SUV39H2 Recombinant Rabbit Monoclonal Antibody [JG36-55] (ET7108-37)
Immunogen
Synthetic peptide within human suv39h2 aa 361-410 / 410.
Host
Rabbit
Positive Control
U-2 OS cell lysate, A549 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG36-55
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,000
-
IP
-
1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SUV39H2
SYNONYMS
FLJ23414 antibody;H3 K9 HMTase 2 antibody;H3-K9-HMTase 2 antibody;Histone H3 K9 methyltransferase 2 antibody;Histone H3-K9 methyltransferase 2 antibody;Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody;Histone lysine N methyltransferase SUV39H2 antibody;Histone-lysine N-methyltransferase SUV39H2 antibody;KMT1B antibody;Lysine N methyltransferase 1B antibody;Lysine N-methyltransferase 1B antibody;sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody;Su(var)3 9 Drosophila homolog of 2 antibody;Su(var)3 9 homolog 2 antibody;Su(var)3-9 homolog 2 antibody;Suppressor of variegation 3 9 homolog 2 antibody;Suppressor of variegation 3-9 homolog 2 antibody;Suv39h2 antibody;SUV92_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
CITATIONS
-
Ma Runze , Zhang Yan , Zhang Jing , et al
Targeting pericentric non-consecutive motifs for heterochromatin initiation
-
WB
-
IF
-
Human
-
Mouse
-
Citation
-
-
Jiemin Wong,et al
An H3K14 ubiquitination-dependent SUV39H recruitment and activation is uniquely required for mammalian pericentromeric heterochromatin formation
-
WB
-
Human
-
Citation
-
