Tartrate-resistant acid phosphatase type 5 Recombinant Rabbit Monoclonal Antibody [JE35-95] (HA721897)
Catalog# HA721897
Rabbit Monoclonal to Tartrate-resistant acid phosphatase type 5
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Tartrate-resistant acid phosphatase type 5 on different lysates with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody (HA721897) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SK-MEL-28 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721897) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: SK-MEL-28 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721897) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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