TATA binding protein TBP Rabbit Polyclonal Antibody (HA500518)
Catalog# HA500518
Rabbit Polyclonal to TATA binding protein TBP
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: Hela cell lysate (20 µg/Lane)
Lane 2: HCT116 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: MCF-7 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: C2C12 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 40/36 kDa
Exposure time: 3 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500518) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
TATA binding protein TBP Rabbit Polyclonal Antibody (HA500518)
Immunogen
Recombinant protein within human tbp aa 140-339 / 339.
Host
Rabbit
Positive Control
Hela cell lysate, HCT116 cell lysate, 293T cell lysate, HepG2 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human bladder carcinoma tissue, human testis tissue, human colon tissue, mouse stomach tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 38 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TATA binding protein TBP
SYNONYMS
GTF2D antibody;GTF2D1 antibody;HDL4 antibody;MGC117320 antibody;MGC126054 antibody;MGC126055 antibody;SCA17 antibody;TATA binding factor antibody;TATA box factor antibody;TATA sequence binding protein antibody;TATA sequence-binding protein antibody;TATA-binding factor antibody;TATA-box binding protein N-terminal domain antibody;TATA-box factor antibody;TATA-box-binding protein antibody;TBP antibody;TBP_HUMAN antibody;TFIID antibody;Transcription initiation factor TFIID TBP subunit antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. This DNA sequence is found about 30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP is a member of a small gene family of TBP-related factors. The first TBP-related factor (TRF/TRF1) was identified in the fruit fly Drosophila, but appears to be fly or insect-specific. Subsequently TBPL1/TRF2 was found in the genomes of many metazoans, whereas vertebrate genomes encode a third vertebrate family member, TBPL2/TRF3. In specific cell types or on specific promoters TBP can be replaced by one of these TBP-related factors, some of which interact with the TATA box similarly to TBP.
CITATIONS
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Yan Luo,et al
Luteolin blocks dopaminergic neuron degeneration and shifts microglial M1/M2 polarization by inhibiting Toll like receptor 4
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WB
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Mouse
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Citation
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Xie Yangzhi,et al
Metabotropic Glutamate Receptor 8 Suppresses M1 Polarization in Microglia by Alleviating Endoplasmic Reticulum Stress and Mitochondrial Dysfunction
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WB
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Mouse
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Citation
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Yangzhi Xie,et al
Luteolin Mitigates Dopaminergic Neuron Degeneration and Restrains Microglial M1 Polarization by Inhibiting Toll Like Receptor 4
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WB
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Mouse
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Citation
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