TEAD1 Recombinant Rabbit Monoclonal Antibody [JE52-71] (ET7111-04)
Catalog# ET7111-04
Rabbit Monoclonal to TEF1
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
All lanes: Western blot analysis of TEF1 with anti-TEF1 antibody [JE52-71] (ET7111-04) at 1:500 dilution.
Lane 1: Wild-type ct26.wt whole cell lysate.
Lane 2: TEF1 knockout ct26.wt whole cell lysate.
ET7111-04 was shown to specifically react with TEF1 in wild-type ct26.wt cells. No band was observed when TEF1 knockout samples were tested. Wild-type and TEF1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-TEF1 antibody (ET7111-04, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TEAD1 Recombinant Rabbit Monoclonal Antibody [JE52-71] (ET7111-04)
Immunogen
Synthetic peptide within human tead1 aa 1-50 / 426.
Host
Rabbit
Positive Control
A431 cell lysate, A549 cell lysate, HeLa cell lysate, Mouse embryo tissue lysate, Mouse skeletal muscle tissue lysate, Rat embryo tissue lysate, Rat skeletal muscle tissue lysate, human colon cancer tissue, human testis tissue, A549, MG-63, SiHa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE52-71
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TEF1
SYNONYMS
AA antibody;Atrophia areata peripapillary chorioretinal degeneration antibody;NTEF 1 antibody;NTEF-1 antibody;NTEF1 antibody;Protein GT IIC antibody;Protein GT-IIC antibody;REF 1 antibody;REF1 antibody;SV40 transcriptional enhancer factor antibody;TCF 13 antibody;TCF-13 antibody;TCF13 antibody;TEA domain family member antibody;TEA domain family member 1 (SV40 transcriptional enhancer factor) antibody;TEA domain family member 1 antibody;TEAD 1 antibody;TEAD 1 protein antibody;TEAD-1 antibody;TEAD1 antibody;TEAD1 protein antibody;TEAD1_HUMAN antibody;TEF 1 antibody;TEF1 antibody;Transcription factor 13 (SV40 transcriptional enhancer factor) antibody;Transcription factor 13 antibody;Transcriptional enhancer factor 1 antibody;Transcriptional enhancer factor TEF-1 antibody;Transcriptional enhancer factor TEF1 antibody
TISSUE SPECIFICITY
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
CITATIONS
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Li Kehan,et al
Hippo/YAP1 promotes osteoporotic mice bone defect repair via the activating of Wnt signaling pathway
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WB
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Mouse
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Citation
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