TLR2 Recombinant Rabbit Monoclonal Antibody [JM22-41] (ET1705-92)
Catalog# ET1705-92
Rabbit Monoclonal to TLR2
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WB
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FC
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: THP-1 cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 90 kDa
Observed band size: 80-90 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-92) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TLR2 Recombinant Rabbit Monoclonal Antibody [JM22-41] (ET1705-92)
Immunogen
Recombinant protein within human tlr2 aa 21-588 (extracellular).
Host
Rabbit
Positive Control
THP-1 cell lysate, HL-60 cell lysate, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM22-41
RRID
APPLICATION DILUTION
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WB
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1:1,000
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FC
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1:50-1:100
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IF-Cell
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 90 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TLR2
SYNONYMS
CD282 antibody;CD282 antigen antibody;TIL 4 antibody;TIL4 antibody;TLR 2 antibody;TLR2 antibody;TLR2_HUMAN antibody;Toll like receptor 2 antibody;Toll like receptor 2 precursor antibody;Toll-like receptor 2 antibody;Toll/interleukin 1 receptor like 4 antibody;Toll/interleukin 1 receptor like protein 4 antibody;Toll/interleukin receptor like protein 4 antibody;Toll/interleukin-1 receptor-like protein 4 antibody
SEQUENCE SIMILARITIES
Belongs to the Toll-like receptor family.
TISSUE SPECIFICITY
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
POST-TRANSLATIONAL MODIFICATION
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.; Ubiquitinated at Lys-754 by PPP1R11, leading to its degradation. Deubiquitinated by USP2 (By similarity).
SUBCELLULAR LOCATION
Membrane, Phagosome membrane, Membrane raft.
FUNCTION
The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes. TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation.
CITATIONS
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Ouyang, A., Wang, H., Su, J., & Liu, X.
Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream (Megalobrama amblycephala) Macrophages
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WB
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Blunt snout bream
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M.amblycephala
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Citation
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Efficacy of Sishen Wan on dinitrobenzene sulfonic acid-induced ulcerative colitis and its effect on toll-like receptor 2/interleukin-1 receptor-associated kinase-4/nuclear factor-κB signal pathway
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WB
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IHC
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Mouse
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Citation
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Kaihuan Yu,et al
Sparstolonin B potentiates the antitumor activity of nanovesicle-loaded drugs by suppressing the phagocytosis of macrophages in vivo
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WB
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Human
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Citation
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