TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77] (ET7108-80)
Catalog# ET7108-80
Rabbit Monoclonal to TNPO3
-
WB
-
IHC-P
-
FC
-
IF-Tissue
-
Human
-
Mouse
-
Rat
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HL-60 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Ramos cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 7: Rat pancreas tissue lysate (40 µg/Lane)
Predicted band size: 104 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-80) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77] (ET7108-80)
Immunogen
Recombinant protein within human tnpo3 aa 830-923 / 923.
Host
Rabbit
Positive Control
K-562 cell lysate, HL-60 cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, mouse kidney tissue, rat kidney tissue, human small intestine tissue, K-562.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG38-77
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:1,000
-
IF-Tissue
-
1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 104 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TNPO3
SYNONYMS
TNPO 3 antibody;Imp12 antibody;Importin 12 antibody;Importin-12 antibody;IPO12 antibody;MTR10A antibody;TNPO3 antibody;TNPO3_HUMAN antibody;Transportin 3 antibody;Transportin SR antibody;Transportin-3 antibody;Transportin-SR antibody;TRN SR antibody;TRN-SR antibody;TRN-SR2 antibody;TRNSR antibody
TISSUE SPECIFICITY
Expressed in skeletal muscle.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
The Importin complex consists of Importin-α and Importin-β proteins which assist in transport of arginine- or serine-rich (SR) proteins across the nucleus. Importin-12, also known as Transportin-3 or Transportin-SR, is a member of the Importin-β family and functions as a nuclear transport receptor for serine/arginine-rich proteins. Through recognition of phosphorylated RS domains, Importin-12 mediates the nuclear import of several SR proteins, such as splicing factors SFRS1 and SFRS2. By regulating the nucleocytoplasmic transport of these and other SR mRNA splicing factors, Importin-12 controls their access to mRNA and, therefore, acts a transcriptional regulator.
CITATIONS
-
Zou, J., Yu, L., Zhu, Y., Yang, S., Zhao, J., Zhao, Y., Jiang, M., Xie, S., Liu, H., Zhao, C., & Zhou, H.
Transportin-3 Facilitates Uncoating of Influenza A Virus
-
KO
-
ICC
-
Human
-
Citation
-
