Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02] (ET1706-35)
Catalog# ET1706-35
Rabbit Monoclonal to Transglutaminase 2
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Knockout (KO)
All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (ET1706-35) at 1:1,000 dilution.
Lane 1: Wild-type MDCK whole cell lysate.
Lane 2: TGM2 knockout MDCK whole cell lysate.
ET1706-35 was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (ET1706-35, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (ET1706-35) at 1:1,000 dilution.
Lane 1: Wild-type MDCK whole cell lysate.
Lane 2: TGM2 knockout MDCK whole cell lysate.
ET1706-35 was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (ET1706-35, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).