TRPM2 Rabbit Polyclonal Antibody (HA500437)
Catalog# HA500437
Rabbit Polyclonal to TRPM2
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WB
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FC
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Human
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Mouse
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Rat
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 171 kDa
Observed band size: 171/120 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
TRPM2 Rabbit Polyclonal Antibody (HA500437)
Immunogen
Synthetic peptide within mouse trpm2 aa 100-150 / 1503 (cytoplasmic).
Host
Rabbit
Positive Control
THP-1 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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FC
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 171 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
TRPM2
SYNONYMS
EREG 1 antibody;EREG1 antibody;Estrogen responsive element associated gene 1 antibody;Estrogen responsive element associated gene 1 protein antibody;Estrogen-responsive element-associated gene 1 protein antibody;KNP 3 antibody;KNP3 antibody;Long transient receptor potential channel 2 antibody;LTRPC 2 antibody;LTrpC-2 antibody;LTrpC2 antibody;MGC133383 antibody;NUDT9H antibody;NUDT9L1 antibody;putative Ca2+ channel protein antibody;putative Ca2+ channel protein antibody;Transient receptor potential cation channel subfamily M member 2 antibody;Transient receptor potential channel 7 antibody;TRPC 7 antibody;TrpC7 antibody;TRPM 2 antibody;Trpm2 antibody;TRPM2_HUMAN antibody
SUBCELLULAR LOCATION
Lysosome, cell membrane, perikaryon, cell projection, cytoplasmic vesicle.
FUNCTION
Transient receptor potential cation channel, subfamily M, member 2, also known as TRPM2, is a protein that in humans is encoded by the TRPM2 gene. The TRPM2 gene is highly expressed in the brain and was implicated by both genetic linkage studies in families and then by case control or trio allelic association studies in the genetic aetiology of bipolar affective disorder (Manic Depression). The physiological role of TRPM2 is not well understood. It was shown to be involved in insulin secretion.[5][11] In the immune cells it mediates parts of the responses to TNF-alpha. A role has been suggested for TRPM2 in activation of NLRP3 inflammasome, the dysregulation of which is strongly associated with a number of auto inflammatory and metabolic diseases, such as gout, obesity and diabetes.[13] In the brain it is involved in the toxicity of amyloid beta, a protein associated with Alzheimer's disease.[14] In 2016, TRPM2 channel was strongly implicated in the detection of non-painful warm stimuli. Chun-Hsiang Tan and Peter McNaughton studied the responses of actual sensory neurons to thermal stimuli, then used an RNA-sequencing strategy to identify TRPM2 as genetically required for warmth detection in the non-noxious range of 33–38 °C.
CITATIONS
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Danxi Zheng, Siyu Long, Mingrong Xi
Identification of TRPM2 as a Potential Therapeutic Target Associated with Immune Infiltration: A Comprehensive Pan-Cancer Analysis and Experimental Verification in Ovarian Cancer
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IHC
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Human
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Citation
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TRPM2 protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction via modulating autophagy
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IHC-P
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Human
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Citation
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