UBE3A Recombinant Rabbit Monoclonal Antibody [JE32-48] (HA721921)
Catalog# HA721921
Rabbit Monoclonal to UBE3A
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of UBE3A on different lysates with Rabbit anti-UBE3A antibody (HA721921) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721921) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721921) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.