UQCRC2 Rabbit Polyclonal Antibody (ER1803-08)
Catalog# ER1803-08
Rabbit Polyclonal to UQCRC2
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: L-929 cell lysate (10 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: Rat heart tissue lysate (20 µg/Lane)
Lane 7: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 48 kDa
Observed band size: 40 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-08) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
UQCRC2 Rabbit Polyclonal Antibody (ER1803-08)
Immunogen
Recombinant protein within human uqcrc2 aa 90-370.
Host
Rabbit
Positive Control
HepG2 cell lysate, Jurkat cell lysate, L-929 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat heart tissue lysate, rat kidney tissue lysate, HepG2, LOVO, SiHa, rat heart tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
UQCRC2
SYNONYMS
Complex III subunit 2 antibody;Core protein II antibody;Cytochrome b c1 complex subunit 2 mitochondrial antibody;Cytochrome b-c1 complex subunit 2 antibody;EC 1.10.2.2 antibody;MC3DN5 antibody;mitochondrial antibody;QCR2 antibody;QCR2_HUMAN antibody;Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody;Ubiquinol cytochrome c reductase core protein II antibody;Ubiquinol-cytochrome-c reductase complex core protein 2 antibody;UQCR2 antibody;Uqcrc2 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b associates with cytochrome c subunit 1 and the Rieske protein to form complex III (also designated cytochrome bc1 complex), which is involved in cellular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also referred to as Rieske iron-sulfur protein, represents an important subunit of complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 respiratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c reductase complex core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex.
CITATIONS
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Knockdown of PGC1α suppresses dysplastic oral keratinocytes proliferation through reprogramming energy metabolism
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WB
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IHC
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Human
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Citation
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Xie, X., Zhou, J., Hu, L., Shu, R., Zhang, M., Sun, L., Wu, F., Fu, Z., & Li, Z.
Oral exposure to a hexafluoropropylene oxide trimer acid (HFPO-TA) disrupts mitochondrial function and biogenesis in mice
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WB
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Mouse
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Citation
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