VDAC1 Rabbit Polyclonal Antibody (R1307-1)
Catalog# R1307-1
Rabbit Polyclonal to VDAC1
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WB
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IF-Cell
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IHC-P
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Human
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Zebrafish
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: Raji cell lysate
Lane 5: HepG2 cell lysate
Lane 6: SW480 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
VDAC1 Rabbit Polyclonal Antibody (R1307-1)
Immunogen
Synthetic peptide within human vdac1 aa 188-231.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, Raji cell lysate, HepG2 cell lysate, SW480 cell lysate, A549 cell lysate, human brain tissue, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
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IF-Cell
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
VDAC1
SYNONYMS
N2441 antibody;OMP2 antibody;POR1 antibody;hVDAC1 antibody;MGC111064 antibody;Mitochondrial Porin antibody;Outer mitochondrial membrane protein porin 1 antibody;Plasmalemmal porin antibody;Porin 31HL antibody;Porin 31HM antibody;VDAC antibody;VDAC-1 antibody;Vdac1 antibody;VDAC1_HUMAN antibody;Voltage dependent anion channel 1 antibody;Voltage dependent anion selective channel protein 1 antibody;Voltage-dependent anion-selective channel protein 1 antibody;YNL055C antibody;YNL2441C antibody
SEQUENCE SIMILARITIES
Belongs to the eukaryotic mitochondrial porin family.
TISSUE SPECIFICITY
Heart, liver and skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.; Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
SUBCELLULAR LOCATION
Mitochondrial membrane, cell membrane
FUNCTION
Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis.
CITATIONS
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Tao, M., Zhu, W., Han, H., Liu, S., Liu, A., Li, S., Fu, H., & Tian, J.
Mitochondrial proteomic analysis reveals the regulation of energy metabolism and reactive oxygen species production in Clematis terniflora DC. leaves under high-level UV-B radiation followed by dark treatmenttreatment
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WB
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Citation
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