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Vimentin Mouse Monoclonal Antibody [D4-B11] (EM0401)

Price: Regular price $350.00

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Catalog# EM0401

Mouse Monoclonal to Vimentin

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IF-Tissue

  • IHC-Fr

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

Select your chosen country/region

Specifications

Product Type

Mouse monoclonal primary

Product Name

Vimentin Mouse Monoclonal Antibody [D4-B11] (EM0401)

Immunogen

Synthetic peptide within c-terminal human vimentin.

Host

Mouse

Positive Control

HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, Mouse embryonic stem cell lysate, Hela, human kidney tissue, human colon carcinoma tissue, human stomach carcinoma tissue, human tonsil tissue, human skin tissue, human liver tissue, human appendix tissue, human endometrium tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

D4-B11

RRID

AB_3068733

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:500-1:1,000

  • IF-Tissue

  • 200-400

  • IHC-Fr

  • 1:100

  • IP

  • Use at an assay dependent concentration

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 54 kDa

Isotype

IgG1

TARGET

UNIPROT #

P08670

PROTEIN NAME

Vimentin

SYNONYMS

CTRCT30 antibody;Epididymis luminal protein 113 antibody;FLJ36605 antibody;HEL113 antibody;VIM antibody;VIME_HUMAN antibody;Vimentin antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

POST-TRANSLATIONAL MODIFICATION

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells[6] as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

CITATIONS

  • Tao Jing,et al

    A nano-platform combats the "attack" and "defense" of cytoskeleton to block cascading tumor metastasis

  • He, X., Ma, Y., Huang, Z., Wang, G., Wang, W., Zhang, R., Guo, G., Zhang, X., Wen, Y., & Zhang, L.

    SERPINB5 is a prognostic biomarker and promotes proliferation, metastasis and epithelial-mesenchymal transition (EMT) in lung adenocarcinoma

  • Zhong Xin,et al

    Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R

  • Liu Yang,et al

    RGS10 deficiency facilitates distant metastasis by inducing epithelial-mesenchymal transition in breast cancer

  • Wang, Z., Li, Y., Zhao, W., Jiang, S., Huang, Y., Hou, J., Zhang, X., Zhai, Z., Yang, C., Wang, J., Zhu, J., Pan, J., Jiang, W., Li, Z., Ye, M., Tan, M., Jiang, H., & Dang, Y.

    Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells

  • Jin, L., Yu, B., Liu, G., Nie, W., Wang, J., Chen, J., Xiao, L., Xia, H., Han, F., & Yang, Y.

    Mitophagy induced by UMI-77 preserves mitochondrial fitness in renal tubular epithelial cells and alleviates renal fibrosis

  • Liu Yangfan,et al

    Fractionated irradiation induces radioresistant oral carcinoma cells with enhanced malignant phenotypes

  • He, S., Li, S., Guo, J., Zeng, X., Liang, D., Zhu, Y., Li, Y., Yang, D., & Zhao, X.

    CD166-specific CAR-T cells potently target colorectal cancer cells

  • Minglu Zhou,et al

    Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition

  • Sclareol attenuates liver fibrosis through SENP1‐mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling

  • Meng, Y., Hu, X., Li, S., Zeng, X., Qiu, L., Wei, M., Wang, Z., & Han, J.

    miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1. BMC cancer, 20(1), 1028.

  • TRPM2 protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction via modulating autophagy

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