Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30] (ET1706-33)
Catalog# ET1706-33
Rabbit Monoclonal to Wnt5a
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WB
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IHC-P
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Human
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30] (ET1706-33)
Immunogen
Synthetic peptide within human wnt5a aa 331-380 / 380.
Host
Rabbit
Positive Control
HeLa cell lysates, human lung cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU30-30
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Wnt5a
SYNONYMS
hWNT 5A antibody;hWNT5A antibody;Protein Wnt 5a antibody;Protein Wnt-5a antibody;Protein Wnt5a antibody;Wingless type MMTV integration site family member 5A antibody;Wnt 5a antibody;WNT 5A protein antibody;WNT 5A protein precursor antibody;WNT5A antibody;WNT5A protein precursor antibody;WNT5A_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the Wnt family.
TISSUE SPECIFICITY
Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected in neonate heart and lung.
POST-TRANSLATIONAL MODIFICATION
Glycosylation is necessary for secretion but not for activity.; Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.; Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.
SUBCELLULAR LOCATION
Secreted, extracellular space, extracellular matrix.
FUNCTION
The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. At least five distinct Wnt-5a transcripts are observed, which are due to transcript variability 5-prime to the initiation methionine. In situ hybridization detects a complex spatial and temporal pattern of Wnt-5a in the mouse, including expression in the developing central nervous system, limbs, facial processes, and the posterior region of the fetus. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and represents an in vivo regulator of hematopoietic stem cell function in the human.
CITATIONS
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Fei Zhou,Jihong Sun and Xiaoming Yang
WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling.
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WB
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breast cell lines
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Citation
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Fangquan Wu ,et al
Reduced Proline-Rich Tyrosine Kinase 2 Promotes Tumor Metastasis by Activating Epithelial–Mesenchymal Transition in Colorectal Cancer
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WB
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Human
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Citation
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