Class II HDAC Antibody Sampler Kit (HAK21073)
Catalog# HAK21073
Reacts with
-
Human
Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si HDAC6 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 160 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si HDAC6 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 160 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.