Alzheimer's Disease Antibody Sampler Kit (HAK21109)
Catalog# HAK21109
Reacts with
-
Human
Western blot analysis of Amyloid Precursor Protein on different lysates with Rabbit anti-Amyloid Precursor Protein antibody (HA722139) at 1/1,000 dilution.
Lane 1: PC-3 cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse skeletal muscle tissue lysate (negative)
Lane 5: Rat brain tissue lysate
Lane 6: Rat skeletal muscle tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722139) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: PC-3 cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse skeletal muscle tissue lysate (negative)
Lane 5: Rat brain tissue lysate
Lane 6: Rat skeletal muscle tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722139) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.