BACE1 Recombinant Rabbit Monoclonal Antibody [PSH05-19] (HA722244)
Catalog# HA722244
Rabbit Monoclonal to BACE1
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56-80 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722244) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
BACE1 Recombinant Rabbit Monoclonal Antibody [PSH05-19] (HA722244)
Immunogen
Recombinant fragment within mouse bace1 aa 50-350.
Host
Rabbit
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH05-19
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IHC-Fr
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1:200
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IF-Tissue
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 56 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
BACE1
SYNONYMS
APP beta secretase antibody;Asp 2 antibody;ASP2 antibody;Aspartyl protease 2 antibody;BACE 1 antibody;BACE antibody;BACE1 antibody;BACE1_HUMAN antibody;Beta secretase 1 antibody;Beta secretase antibody;Beta site amyloid beta A4 precursor protein cleaving enzyme antibody;Beta site amyloid precursor protein cleaving enzyme 1 antibody;Beta site amyloid precursor protein cleaving enzyme antibody;Beta site APP cleaving enzyme 1 antibody;Beta site APP cleaving enzyme antibody;Beta-secretase 1 antibody;Beta-site amyloid precursor protein cleaving enzyme 1 antibody;Beta-site APP cleaving enzyme 1 antibody;FLJ90568 antibody;HSPC104 antibody;Memapsin 2 antibody;Memapsin-2 antibody;Memapsin2 antibody;Membrane associated aspartic protease 2 antibody;Membrane-associated aspartic protease 2 antibody;Transmembrane aspartic proteinase Asp2 antibody
SUBCELLULAR LOCATION
Cell membrane, Golgi apparatus, trans-Golgi network, Endoplasmic reticulum, Endosome, Cell surface, Cytoplasmic vesicle membrane, Membrane raft, Lysosome, Late endosome, Early endosome, Recycling endosome, Cell projection, axon, dendrite.
FUNCTION
Beta-secretase 1, also known as beta-site amyloid precursor protein cleaving enzyme 1, beta-site APP cleaving enzyme 1 (BACE1), membrane-associated aspartic protease 2, memapsin-2, aspartyl protease 2, and ASP2, is an enzyme that in humans is encoded by the BACE1 gene. Expression of BACE1 is observed mainly in neurons. BACE1 is an aspartic acid protease important in the formation of myelin sheaths in peripheral nerve cells: in mice the expression of BACE1 is high in the postnatal stages, when myelination occurs. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer, its cytoplasmic tail is required for the correct maturation and an efficient intracellular trafficking, but doesn't affect the activity. It is produced as a pro-enzyme, the endoproteolitc removal occurs after BACE leaves Endoplasmic reticulum, in the Golgi apparatus. In addition the pro-peptide receives additional sugars to increase the molecular mass. and the tail became a palmitoylated. The BACE1 expression is influenced by the inflammatory state: during AD the cytokines reduce the PPAR1 an inhibitor of BACE1 mRNA).
CITATIONS
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Zixiang Li,et al
Avicularin inhibits ferroptosis and improves cognitive impairments in Alzheimer's disease by modulating the NOX4/Nrf2 axis
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WB
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Mouse
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Citation
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