CRL4/CRBN Targeted Protein Degradation Complex Antibody Sampler Kit (HAK21095)
Catalog# HAK21095
Reacts with
-
Human
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721457) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HeLa cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HeLa cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.