Cell Cycle Phase Determination Antibody Kit (K2004)
Catalog# K2004
Reacts with
-
Human
Western blot analysis of Phospho-Histone H3 (S10) on different lysates with Rabbit anti-Phospho-Histone H3 (S10) antibody (ET1601-30) at 1/5,000 dilution.
Lane 1: HeLa whole cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 50nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 whole cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Lane 5: C6 whole cell lysate (20 µg/Lane)
Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-30) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa whole cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 50nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 whole cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Lane 5: C6 whole cell lysate (20 µg/Lane)
Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane)
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-30) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.