Skip to content

Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1702-31

Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Thymidine Kinase 1 aa 2-49 / 234.

Species Reactivity

Human

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 25 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JF0970

RRID

AB_3070293

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

Target

Function

Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer.

Background References

1. Li Z et al. Pan-cancer analysis reveals that TK1 promotes tumor progression by mediating cell proliferation and Th2 cell polarization. Cancer Cell Int. 2024 Sep

2. Bitter EE et al. TK1 expression influences pathogenicity by cell cycle progression, cellular migration, and cellular survival in HCC 1806 breast cancer cells. PLoS One. 2023 Nov

Sequence Similarity

Belongs to the thymidine kinase family.

Post-translational Modification

Phosphorylated on Ser-13 in mitosis.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P04183 Human

Synonyms

cytosolic antibody

KITH_HUMAN antibody

Thymidine kinase 1 antibody

Thymidine kinase 1 soluble antibody

Thymidine kinase 1 soluble isoform antibody

Thymidine kinase antibody

Thymidine kinase cytosolic antibody

TK 1 antibody

TK1 antibody

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si Thymidine Kinase 1 / TK1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 45 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si Thymidine Kinase 1 / TK1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 45 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation