Parkinson's Research Antibody Sampler Kit (K2003)
Catalog# K2003
Reacts with
-
Human
-
Mouse
Western blot analysis of PARK7/DJ1 on different lysates with Rabbit anti-PARK7/DJ1 antibody (ET1611-45) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-45) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-45) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.