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PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]

Usd: 385

Specification

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Catalog# HA723021

PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PINK1 Recombinant Rabbit Monoclonal Antibody [PSH08-69]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human PINK1 aa 94-581 / 581.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr

Target Molecular Weight

Predicted band size: 63 kDa

Positive Control

HeLa treated with 10μM CCCP for 24 hours cell lysate, MDA-MB-231 cell lysate, human kidney tissue, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH08-69

Reactivity Data

WB IHC-P IHC-Fr
Human
Tested Tested
Tested Tested
Mouse
Tested
Tested Tested
Tested Tested
Rat
Not Recommended
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Pig
Published
Published
Published

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:1,000

  • IHC-Fr

  • 1:500

Target

Function

A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons.

Background References

1. Quinn PMJ et al. PINK1/PARKIN signalling in neurodegeneration and neuroinflammation. Acta Neuropathol Commun. 2020 Nov

2. Han R et al. PINK1-PRKN mediated mitophagy: differences between in vitro and in vivo models. Autophagy. 2023 May

Subcellular Location

Mitochondrion outer membrane, Mitochondrion inner membrane, Cytoplasm, cytosol.

UNIPROT

SwissProt: Q9BXM7 Human

SwissProt: Q99MQ3 Mouse

SwissProt: B5DFG1 Rat

Synonyms

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

PARK6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

PINK1 antibody

PINK1_HUMAN antibody

Protein kinase BRPK antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate<br />Lane 3: MDA-MB-231 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 10 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (HA723021) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate
    Lane 3: MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 10 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723021) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PINK1 antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PINK1 antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PINK1 antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PINK1 antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723021" style="font-weight: bold;text-decoration: underline;">HA723021</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PINK1 antibody (HA723021) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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