Parkin Recombinant Rabbit Monoclonal Antibody [JF82-09] (ET1702-60)

UNIPROT #

O60260

PROTEIN NAME

Parkin

SYNONYMS

AR JP antibody;E3 ubiquitin ligase antibody;E3 ubiquitin protein ligase parkin antibody;E3 ubiquitin-protein ligase parkin antibody;FRA6E antibody;LPRS 2 antibody;LPRS2 antibody;PARK 2 antibody;Park2 antibody;Parkin 2 antibody;Parkinson disease (autosomal recessive juvenile) 2 antibody;Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody;Parkinson disease protein 2 antibody;Parkinson juvenile disease protein 2 antibody;Parkinson protein 2 E3 ubiquitin protein ligase antibody;Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody;PDJ antibody;PRKN 2 antibody;PRKN antibody;PRKN2 antibody;PRKN2_HUMAN antibody;Ubiquitin E3 ligase PRKN antibody

SEQUENCE SIMILARITIES

Belongs to the RBR family. Parkin subfamily.

TISSUE SPECIFICITY

Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level).

POST-TRANSLATIONAL MODIFICATION

Auto-ubiquitinates in an E2-dependent manner leading to its own degradation. Also polyubiquitinated by RNF41 for proteasomal degradation.; S-nitrosylated. The inhibition of PRKN ubiquitin E3 ligase activity by S-nitrosylation could contribute to the degenerative process in PD by impairing the ubiquitination of PRKN substrates.; Phosphorylation at Ser-65 by PINK1 contributes to activate PRKN activity. It is however not sufficient and requires binding to phosphorylated ubiquitin as well.

SUBCELLULAR LOCATION

Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse.

FUNCTION

Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus. The wild type Parkin gene, which maps to human chromosome 6q25.2-27, encodes a 465 amino acid full-length protein that is expressed as multiple isoforms. Mutations in the Parkin gene are responsible for autosomal recessive juvenile Parkinson's disease and commonly involve deletions of exons 3-5. In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues. Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons. The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution.