Parkin Recombinant Antibody [PSH08-11] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Catalog# HA610448
Parkin Recombinant Antibody [PSH08-11] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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IF-Tissue
-
FC(Intra)
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Parkin Recombinant Antibody [PSH08-11] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human Parkin aa 1-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue, FC(Intra)
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
SH-SY5Y (Human neuroblastoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH08-11
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:5,000
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IF-Cell
-
1:100
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IHC-P
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1:2,000
-
IF-Tissue
-
1:500
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FC(Intra)
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1:1,000
Target
Function
Parkin is a 465-amino acid residue E3 ubiquitin ligase, a protein that in humans and mice is encoded by the PARK2 gene. Parkin plays a critical role in ubiquitination – the process whereby molecules are covalently labelled with ubiquitin (Ub) and directed towards degradation in proteasomes or lysosomes. Ubiquitination involves the sequential action of three enzymes. First, an E1 ubiquitin-activating enzyme binds to inactive Ub in eukaryotic cells via a thioester bond and mobilises it in an ATP-dependent process. Ub is then transferred to an E2 ubiquitin-conjugating enzyme before being conjugated to the target protein via an E3 ubiquitin ligase. There exists a multitude of E3 ligases, which differ in structure and substrate specificity to allow selective targeting of proteins to intracellular degradation.
Background References
1. Quinn PMJ et al. PINK1/PARKIN signalling in neurodegeneration and neuroinflammation. Acta Neuropathol Commun. 2020 Nov
2. Li J et al. PINK1/Parkin-mediated mitophagy in neurodegenerative diseases. Ageing Res Rev. 2023 Feb
Subcellular Location
Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse.
Synonyms
AR JP antibody
E3 ubiquitin ligase antibody
E3 ubiquitin protein ligase parkin antibody
E3 ubiquitin-protein ligase parkin antibody
FRA6E antibody
LPRS 2 antibody
LPRS2 antibody
PARK 2 antibody
Park2 antibody
Parkin 2 antibody
ExpandImages
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Western blot analysis of Parkin on different lysates with Guinea pig anti-Parkin antibody (HA610448) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: COS-1 (African green monkey kidney fibroblast) cell lysate
Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lane 6: Pig brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610448, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: Neuro-2a (Mouse brain neuroblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA610448, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 45 hours at room temperature.
Counterstain: Beta tubulin (HA601187, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610448, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1021, 30 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610448, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1021, 30 minutes at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA610448, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: Neuro-2a (Mouse brain neuroblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA610448 (1/1,000, Red), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 15 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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