Mouse Reactive M1 vs M2 Macrophage Antibody Sampler Kit (HAK21096)
Catalog# HAK21096
Reacts with
-
Mouse
Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane)
Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 102 kDa
Observed band size: 160 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane)
Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 102 kDa
Observed band size: 160 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.